Detection of Campylobacter jejuni Invasion of HEp-2 Cells by Acridine Orange-Crystal Violet Staining

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Assessment of invasion frequencies of cultured HEp-2 cells by clinical isolates of Helicobacter pylori using an acridine orange assay.

AIMS Recent studies suggest that Helicobacter pylori is an invasive enteropathogen. However, the efficiency with which this pathogen invades mammalian cells remains unknown. Therefore, this study was designed to investigate the invasion frequencies of HEp-2 cells by clinical strains of H pylori. METHODS An acridine orange assay and cultured HEp-2 cell monolayers were used to determine the HEp...

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Campylobacter jejuni motility and invasion of Caco-2 cells.

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Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 1997

ISSN: 0074-0276

DOI: 10.1590/s0074-02761997000400012